1. 20x AddScript Enzyme Solution 50 µl
2. 2.5x Buffer 0.5 ml
3. Nuclease-free H20 1.0 ml
AddScript RT-PCR Kit provides sensitive and easy-to-use components which contain
all the reagents for first strand cDNA synthesis and PCR reaction in one-tube reaction continuously.
Especially, thermostable MMLV RTase (RNase H-), hot-start Taq DNA Polymerase and
RNase Inhibitor are included in 20x AddScript Enzyme Solution.
The loading dye and sediment for electrophoresis and dNTP mixture are also included in 2.5x Buffer.