1. 20x AddScript Enzyme Solution 50 µl
2. 2x Reaction Buffer 0.5 ml
3. 10mM dNTP Mixture (2.5mM each) 0.1 ml
4. 10x oligo dT20 0.1 ml
5. 10x random hexamer 0.1 ml
6. Nuclease-free H20 1.0 ml
AddScript cDNA Synthesis Kit provides sensitive and easy-to-use components which
contain all the reagents for first strand cDNA synthesis.
Especially, thermostable MMLV RTase (RNase H-) and RNase Inhibitor are included in
20x AddScript Enzyme Solution.
This kit can be also applied to real-time PCR by fluorescent intercalating dye like SYBR Green I®
and TaqMan® Probe.