1. AddStart Taq DNA Polymerase (2.5 U/µl) 1,000 units, 400 µl
2. 10x Reaction Buffer (without Mg) 1.2 ml x 2 tubes
3. 25mM MgCl2 1.2 ml x 2 tubes
AddStart Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase
derived from the thermophile, Thermus aquaticus, and is a hot-start Taq DNA Polymerase by
specific anti-Taq monoclonal antibody.
AddStart Taq DNA polymerase catalyzes the 5’→3’ synthesis of DNA but has no detectable
3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity,
which results in a 3’-dA overhang on the PCR product.
Especially, this enzyme can be applied to multiplex PCR, allele specific PCR, SNP analysis and
real-time PCR by fluorescent intercalating dye like SYBR Green I® and TaqMan® Probe.