Cat. No.
17001X
Component
1. Add Taq DNA Polymerase (5.0 U/ µl) 1,000 units, 200 µl
2. 10x Reaction Buffer (without Mg) 1.0 ml x 2 tubes
3. 25mM MgCl2 1.0 ml x 2 tubes
Description
Add Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from
the thermophile, Thermus aquaticus.
The molecular weight of the recombinant protein is 94kD.
Add Taq DNA polymerase catalyzes the 5¡¯¡æ3¡¯ synthesis of DNA but has no detectable
3¡¯¡æ5¡¯ proofreading exonuclease activity, and possesses low 5¡¯¡æ3¡¯ exonuclease activity,
which results in a 3¡¯-dA overhang on the PCR product.